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pcyt1a  (Boster Bio)


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    Boster Bio pcyt1a
    Pcyt1a, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcyt1a/pm41043762-171-19-20?v=Boster+Bio
    Average 93 stars, based on 1 article reviews
    pcyt1a - by Bioz Stars, 2026-07
    93/100 stars

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    Image Search Results


    Relative mRNA expression levels of (A) phosphatidylethanolamine N‐methyltransferase ( Pemt ), (B) phospholipase D1 ( Pld1 ), (C) choline dehydrogenase ( Chdh ), (D) choline kinase a ( Chka ), and (E) choline‐phosphate cytidylyltransferase A ( Pcyt1a ) in the liver. Different letters indicate statistically significant differences between the means of groups by one‐way ANOVA with Tukey–Kramer post hoc test. Data presented as means ± SEM, n = 5–8/group.

    Journal: FASEB BioAdvances

    Article Title: Folic Acid Supplementation Attenuates Hepatic Steatosis by Enhancing Choline Availability and Remodeling Fatty Acid Profiles in Mice Fed a High‐Fat Diet

    doi: 10.1096/fba.2025-00251

    Figure Lengend Snippet: Relative mRNA expression levels of (A) phosphatidylethanolamine N‐methyltransferase ( Pemt ), (B) phospholipase D1 ( Pld1 ), (C) choline dehydrogenase ( Chdh ), (D) choline kinase a ( Chka ), and (E) choline‐phosphate cytidylyltransferase A ( Pcyt1a ) in the liver. Different letters indicate statistically significant differences between the means of groups by one‐way ANOVA with Tukey–Kramer post hoc test. Data presented as means ± SEM, n = 5–8/group.

    Article Snippet: An equivalent volume of cDNA was then added to TaqMan Master Mix (Thermo Fisher Scientific) along with gene‐specific probes for phosphatidylethanolamine N‐methyltransferase ( Pemt ; Mm04933134_m1), phospholipase D1 ( Pld1 ; Mm01289339_m1), choline dehydrogenase ( Chdh ; Mm00549261_m1), choline kinase alpha ( Chka ; Mm00442759_m1), choline‐phosphate cytidylyltransferase 1 alpha ( Pcyt1a ; Mm00447774_m1), fatty acid synthase ( Fasn , Mm00662319_m1), stearoyl‐CoA desaturase 1 ( Scd1 , Mm00772290_m1), peroxisome proliferator‐activated receptor alpha ( Ppara , Mm00440939_m1), and carnitine palmitoyltransferase 1A ( Cpt1a , Mm01231183_m1), which were run on a QuantStudio 5 Real‐Time PCR System (Thermo Fisher Scientific).

    Techniques: Expressing

    The protein and messenger RNA (mRNA) expression level of lysophospholipid acyltransferase 3 (LPCAT3) and PCYT1A in the severe and mild groups. a) to c) Representative western blot strips of LPCAT3 and PCYT1A in the severe and mild groups with quantitative analysis (n = 3). d) and e) Quantitative real-time polymerase chain reaction (qRT-PCR) results of LPCAT3 and PCYT1A in the lumbar disc herniation (LDH) patients with mild and severe symptoms with relative quantitative analysis (n = 3). f) and g) Enzyme-linked immunosorbent assay (ELISA) results of LPCAT3 and PCYT1A with quantitative analysis (n = 3). Independent-samples t -test was used for comparison: *p < 0.05, **p < 0.01, ***p < 0.001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Journal: Bone & Joint Research

    Article Title: Unravelling lumbar disc herniation severity beyond MRI

    doi: 10.1302/2046-3758.145.BJR-2024-0071.R1

    Figure Lengend Snippet: The protein and messenger RNA (mRNA) expression level of lysophospholipid acyltransferase 3 (LPCAT3) and PCYT1A in the severe and mild groups. a) to c) Representative western blot strips of LPCAT3 and PCYT1A in the severe and mild groups with quantitative analysis (n = 3). d) and e) Quantitative real-time polymerase chain reaction (qRT-PCR) results of LPCAT3 and PCYT1A in the lumbar disc herniation (LDH) patients with mild and severe symptoms with relative quantitative analysis (n = 3). f) and g) Enzyme-linked immunosorbent assay (ELISA) results of LPCAT3 and PCYT1A with quantitative analysis (n = 3). Independent-samples t -test was used for comparison: *p < 0.05, **p < 0.01, ***p < 0.001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Article Snippet: Primary antibodies, including anti-lysophospholipid acyltransferase 3 (LPCAT3) antibody (1:500, 67882-1-Ig; Proteintech, USA), anti-phosphate cytidylyltransferase 1, choline, alpha (PCYT1A) antibody (1:500, NBP1-84366; NOVUS, USA), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:1,000, 10494-1-AP; Proteintech), were incubated overnight.

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Comparison